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Pan DiMethyl-lysine Rabbit pAb (A5870)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal: - Pan DiMethyl-lysine Rabbit pAb (A5870)

Dot-blot analysis of H3K4me0, H3K4me1, H3K4me2, H3K4me3, H3K9me0, H3K9me1, H3K9me2, H3K9me3, H3K14me0, H3K14me1, H3K14me2, H3K14me3, H3K20me0, H3K20me1, H3K20me2, H3K20me3, H3K27me0, H3K27me1, H3K27me2, H3K27me3, H3K36me0, H3K36me1, H3K36me2, H3K36me3, H3K79me0, H3K79me1, H3K79me2, H3K79me3 using Pan DiMethyl-lysine Rabbit pAb.

ABclonal:Western blot - Pan DiMethyl-lysine Rabbit pAb (A5870)

Western blot analysis of various lysates using Pan DiMethyl-lysine Rabbit pAb (A5870) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Dot Blot - Pan DiMethyl-lysine Rabbit pAb (A5870)

Dot-blot analysis of all sorts of methylation peptides using Pan DiMethyl-lysine antibody (A5870) at 1:1000 dilution.

ABclonal:Immunofluorescence - Pan DiMethyl-lysine Rabbit pAb (A5870)

Immunofluorescence analysis of C6 cells using Pan DiMethyl-lysine Rabbit pAb (A5870) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Pan DiMethyl-lysine Rabbit pAb (A5870)

Immunofluorescence analysis of HeLa cells using Pan DiMethyl-lysine Rabbit pAb (A5870) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Pan DiMethyl-lysine Rabbit pAb (A5870)

Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine Rabbit pAb (A5870) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePan DiMethyl-lysine Rabbit pAb
Catalog No.A5870
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Methylation is a major post-translational modification (PTM) generated by methyltransferase on target proteins, protein methylation plays important regulatory roles in gene expression, protein activity and stability, and signal transduction. Methylation can occur on specific lysine or arginine residues localized within regulatory domains in both histone and nonhistone proteins, thereby allowing distinguished properties of the targeted protein. Lysine can be methylated to different degrees, including mono-, di-, or trimethylation, which reflects its functional diversity and regulatory complexity compared to other PTMs. Lys9 of histone H3 is mono- or di-methylated by G9A/GLP and tri-methylated by SETDB1 to activate transcription. Tumor suppressor p53 is regulated by methylation of at least four sites. p53-mediated transcription is repressed following mono-methylation of p53 at Lys370 by SMYD2; Di-methylation at the same residue further inhibits p53 by preventing association with 53BP1. Concomitant di-methylation at Lys382 inhibits p53 ubiquitination following DNA damage. Di-methylation at Lys373 by G9A/GLP inhibits p53-mediated apoptosis and correlates with tri-methylation of histone H3 Lys9 at the p21 promoter.
ImmunogenA synthetic peptide corresponding to a sequence containing dimethylated K.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW15-60kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa acid extract, C6 acid extract, H3 protein
Cellular location
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal: - Pan DiMethyl-lysine Rabbit pAb (A5870)}

    - Pan DiMethyl-lysine Rabbit pAb (A5870)

    Dot-blot analysis of H3K4me0, H3K4me1, H3K4me2, H3K4me3, H3K9me0, H3K9me1, H3K9me2, H3K9me3, H3K14me0, H3K14me1, H3K14me2, H3K14me3, H3K20me0, H3K20me1, H3K20me2, H3K20me3, H3K27me0, H3K27me1, H3K27me2, H3K27me3, H3K36me0, H3K36me1, H3K36me2, H3K36me3, H3K79me0, H3K79me1, H3K79me2, H3K79me3 using Pan DiMethyl-lysine Rabbit pAb.
    ABclonal:Western blot - Pan DiMethyl-lysine Rabbit pAb (A5870)}

    Western blot - Pan DiMethyl-lysine Rabbit pAb (A5870)

    Western blot analysis of various lysates using Pan DiMethyl-lysine Rabbit pAb (A5870) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Dot Blot - Pan DiMethyl-lysine Rabbit pAb (A5870)}

    Dot Blot - Pan DiMethyl-lysine Rabbit pAb (A5870)

    Dot-blot analysis of all sorts of methylation peptides using Pan DiMethyl-lysine antibody (A5870) at 1:1000 dilution.
    ABclonal:Immunofluorescence - Pan DiMethyl-lysine Rabbit pAb (A5870)}

    Immunofluorescence - Pan DiMethyl-lysine Rabbit pAb (A5870)

    Immunofluorescence analysis of C6 cells using Pan DiMethyl-lysine Rabbit pAb (A5870) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Pan DiMethyl-lysine Rabbit pAb (A5870)}

    Immunofluorescence - Pan DiMethyl-lysine Rabbit pAb (A5870)

    Immunofluorescence analysis of HeLa cells using Pan DiMethyl-lysine Rabbit pAb (A5870) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Pan DiMethyl-lysine Rabbit pAb (A5870)}

    Immunofluorescence - Pan DiMethyl-lysine Rabbit pAb (A5870)

    Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine Rabbit pAb (A5870) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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