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Pan Lactic acid-Lysine Rabbit mAb (A23004)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit mAb (A23004)

Western blot analysis of lysates from MCF7 cells, using Pan Lactic acid-Lysine Rabbit mAb (A23004) at 1:1000 dilution. MCF7 cells were treated by Sodium lactate(100mM) for 24h.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit mAb (A23004)

Western blot analysis of lysates from NIH/3T3 cells, using Pan Lactic acid-Lysine Rabbit mAb (A23004) at 1:1000 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit mAb (A23004)

Western blot analysis of lysates from C6 cells, using Pan Lactic acid-Lysine Rabbit mAb (A23004) at 1:1000 dilution. C6 cells were treated by Sodium lactate(100mM) for 24h.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunoprecipitation - Pan Lactic acid-Lysine Rabbit mAb (A23004)

Immunoprecipitation of Pan Lactic acid-Lysine in 500 µg extracts from MCF7 cells trated by Sodium lactate(200mM，24h) using 2 µg Pan Lactic acid-Lysine Rabbit mAb (A23004). Western blot analysis was performed using Pan Lactic acid-Lysine Rabbit mAb (A23004) at 1:500 dilution.

Overview

Product namePan Lactic acid-Lysine Rabbit mAb
Catalog No.A23004
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59407
Histone lysine lactation (Kla) is a newly discovered histone modification that regulates gene expression in macrophages. In M1 macrophages, lactic acid is derived from incompletely oxidized glucose and then produces lactyl-CoA, which is transferred via acetyltransferase p300 to the lysine tail of the histone. This modification is abundant in gene promoter regions that lack acetylation and are associated with gene expression activation.
ImmunogenA synthetic peptide corresponding to a sequence containing Pan Lactic acid-Lysine.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW15kDa-250kDa/10kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesMCF7 treated by Sodium lactate, NIH/3T3 treated by Sodium lactate, C6 treated by Sodium lactate
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit mAb (A23004)}

    Western blot - Pan Lactic acid-Lysine Rabbit mAb (A23004)

    Western blot analysis of lysates from MCF7 cells, using Pan Lactic acid-Lysine Rabbit mAb (A23004) at 1:1000 dilution. MCF7 cells were treated by Sodium lactate(100mM) for 24h.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit mAb (A23004)}

    Western blot - Pan Lactic acid-Lysine Rabbit mAb (A23004)

    Western blot analysis of lysates from NIH/3T3 cells, using Pan Lactic acid-Lysine Rabbit mAb (A23004) at 1:1000 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit mAb (A23004)}

    Western blot - Pan Lactic acid-Lysine Rabbit mAb (A23004)

    Western blot analysis of lysates from C6 cells, using Pan Lactic acid-Lysine Rabbit mAb (A23004) at 1:1000 dilution. C6 cells were treated by Sodium lactate(100mM) for 24h.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Immunoprecipitation - Pan Lactic acid-Lysine Rabbit mAb (A23004)}

    Immunoprecipitation - Pan Lactic acid-Lysine Rabbit mAb (A23004)

    Immunoprecipitation of Pan Lactic acid-Lysine in 500 µg extracts from MCF7 cells trated by Sodium lactate(200mM，24h) using 2 µg Pan Lactic acid-Lysine Rabbit mAb (A23004). Western blot analysis was performed using Pan Lactic acid-Lysine Rabbit mAb (A23004) at 1:500 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.