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Pan Lactic acid-Lysine Rabbit pAb (A18831)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit pAb (A18831)

Western blot analysis of lysates from MCF7 cells, using Pan Lactic acid-Lysine Rabbit pAb (A18831) at 1:400 dilution. MCF7 cells were treated by Sodium lactate(100mM) for 24h.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit pAb (A18831)

Western blot analysis of lysates from NIH/3T3 cells, using Pan Lactic acid-Lysine Rabbit pAb (A18831) at 1:400 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit pAb (A18831)

Western blot analysis of lysates from C6 cells, using Pan Lactic acid-Lysine Rabbit pAb (A18831) at 1:400 dilution. C6 cells were treated by Sodium lactate(100mM) for 24h.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product namePan Lactic acid-Lysine Rabbit pAb
Catalog No.A18831
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histone lysine lactation (Kla) is a newly discovered histone modification that regulates gene expression in macrophages. In M1 macrophages, lactic acid is derived from incompletely oxidized glucose and then produces lactyl-CoA, which is transferred via acetyltransferase p300 to the lysine tail of the histone. This modification is abundant in gene promoter regions that lack acetylation and are associated with gene expression activation.
ImmunogenA synthetic peptide corresponding to a sequence containing Pan Lactic acid-Lysine.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7 treated by Sodium lactate, NIH/3T3 treated by Sodium lactate, C6 treated by Sodium lactate
Cellular location
Customer validation

IF(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit pAb (A18831)}

    Western blot - Pan Lactic acid-Lysine Rabbit pAb (A18831)

    Western blot analysis of lysates from MCF7 cells, using Pan Lactic acid-Lysine Rabbit pAb (A18831) at 1:400 dilution. MCF7 cells were treated by Sodium lactate(100mM) for 24h.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.
    ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit pAb (A18831)}

    Western blot - Pan Lactic acid-Lysine Rabbit pAb (A18831)

    Western blot analysis of lysates from NIH/3T3 cells, using Pan Lactic acid-Lysine Rabbit pAb (A18831) at 1:400 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.
    ABclonal:Western blot - Pan Lactic acid-Lysine Rabbit pAb (A18831)}

    Western blot - Pan Lactic acid-Lysine Rabbit pAb (A18831)

    Western blot analysis of lysates from C6 cells, using Pan Lactic acid-Lysine Rabbit pAb (A18831) at 1:400 dilution. C6 cells were treated by Sodium lactate(100mM) for 24h.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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