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Pan Mono-Methyl lysine Rabbit pAb (A18293)

Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Pan Mono-Methyl lysine Rabbit pAb (A18293)

Western blot analysis of various lysates using Pan Mono-Methyl lysine Rabbit pAb (A18293) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Western blot - Pan Mono-Methyl lysine Rabbit pAb (A18293)

Western blot analysis of various lysates using Pan Mono-Methyl lysine Rabbit pAb (A18293) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - Pan Mono-Methyl lysine Rabbit pAb (A18293)

Western blot analysis of various lysates using Pan Mono-Methyl lysine Rabbit pAb (A18293) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product namePan Mono-Methyl lysine Rabbit pAb
Catalog No.A18293
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs).
ImmunogenA synthetic peptide corresponding to a sequence containing monomethylated K.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW17-90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, MCF7, NIH/3T3, C6, Mouse liver, Rat liver
Cellular location
Customer validation

WB(Mus musculus, Homo sapiens, C. albicans, Mus musculus)

IP(Homo sapiens)

Co-IP(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Pan Mono-Methyl lysine Rabbit pAb (A18293)}

    Western blot - Pan Mono-Methyl lysine Rabbit pAb (A18293)

    Western blot analysis of various lysates using Pan Mono-Methyl lysine Rabbit pAb (A18293) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Western blot - Pan Mono-Methyl lysine Rabbit pAb (A18293)}

    Western blot - Pan Mono-Methyl lysine Rabbit pAb (A18293)

    Western blot analysis of various lysates using Pan Mono-Methyl lysine Rabbit pAb (A18293) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.
    ABclonal:Western blot - Pan Mono-Methyl lysine Rabbit pAb (A18293)}

    Western blot - Pan Mono-Methyl lysine Rabbit pAb (A18293)

    Western blot analysis of various lysates using Pan Mono-Methyl lysine Rabbit pAb (A18293) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A18293? Please let us know so that we can cite the reference in this datasheet.