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Pan Phospho-Serine/Threonine Rabbit pAb (AP0893)

Publications (11) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Pan Phospho-Serine/Threonine Rabbit pAb (AP0893)

Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-Serine/Threonine Rabbit pAb (AP0893) at 1:400 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s.

ABclonal:Western blot - Pan Phospho-Serine/Threonine Rabbit pAb (AP0893)

Western blot analysis of lysates from HeLa cells using Pan Phospho-Serine/Threonine Rabbit pAb (AP0893) at 1:400 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s.

ABclonal:Western blot - Pan Phospho-Serine/Threonine Rabbit pAb (AP0893)

Western blot analysis of lysates from C6 cells using Pan Phospho-Serine/Threonine Rabbit pAb (AP0893) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s.

Overview

Product namePan Phospho-Serine/Threonine Rabbit pAb
Catalog No.AP0893
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
As a critical post-translational modification, phosphorylation plays important roles in regulating various biological processes, Serine/threonine phosphorylation is an important mechanism that is involved in the regulation of protein function. Protein phosphorylation is the most well-studied post translational modification (PTM), in which a phosphoryl group from adenosine triphosphate (ATP) is covalently attached to a serine (~86%), threonine (~12%), or tyrosine (~2%) by a kinase and removed by a phosphatase. Phosphorylation at other amino acids have also been reported. Phosphorylation can modify protein structure, function, and interactions. As such, phosphorylation plays a critical role in virtually all cellular processes in homeostasis and disease, including signal transduction, cell cycle, differentiation, proliferation, metabolism, motility, and death. Importantly, phosphorylation at different residues can cause different outcomes. For example, RAF1 is a kinase central to the MAPK pathway that is activated when it is phosphorylated at serine (S) or threonine (T) residues S259, S338, S340/341, T491, or S494. However, phosphorylation at S289/296/301 results in the inhibition of RAF1 kinase activity.
ImmunogenA synthetic peptide corresponding to a sequence containing phosphorylated S & T.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW>10kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesC6 treated by Calyculin A, HeLa treated by Calyculin A, NIH/3T3 treated by Calyculin A
Cellular location
Customer validation

WB(Homo sapiens, Other, Mus musculus)

IP(Homo sapiens, Oryctolagus cuniculus)

Pull-down(Arabidopsis thaliana)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Pan Phospho-Serine/Threonine Rabbit pAb (AP0893)}

    Western blot - Pan Phospho-Serine/Threonine Rabbit pAb (AP0893)

    Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-Serine/Threonine Rabbit pAb (AP0893) at 1:400 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 45s.
    ABclonal:Western blot - Pan Phospho-Serine/Threonine Rabbit pAb (AP0893)}

    Western blot - Pan Phospho-Serine/Threonine Rabbit pAb (AP0893)

    Western blot analysis of lysates from HeLa cells using Pan Phospho-Serine/Threonine Rabbit pAb (AP0893) at 1:400 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 45s.
    ABclonal:Western blot - Pan Phospho-Serine/Threonine Rabbit pAb (AP0893)}

    Western blot - Pan Phospho-Serine/Threonine Rabbit pAb (AP0893)

    Western blot analysis of lysates from C6 cells using Pan Phospho-Serine/Threonine Rabbit pAb (AP0893) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 45s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP0893? Please let us know so that we can cite the reference in this datasheet.