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Pan Phospho-T Rabbit pAb (AP1513)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Other (Wide Range Predicted)

ABclonal:Western blot - Pan Phospho-T Rabbit pAb (AP1513)

Western blot analysis of lysates from A-431 cells using Pan Phospho-T Rabbit pAb (AP1513) at 1:900 dilution. A-431 cells were treated by Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Pan Phospho-T Rabbit pAb (AP1513)

Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-T Rabbit pAb (AP1513) at 1:900 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePan Phospho-T Rabbit pAb
Catalog No.AP1513
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic peptide corresponding to a sequence containing phosphorylated T.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW10-250kDa/>10kDa
ReactivityHuman, Mouse, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-431 treated by Calyculin A, NIH/3T3 treated by Calyculin A
Cellular location

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Pan Phospho-T Rabbit pAb (AP1513)}

    Western blot - Pan Phospho-T Rabbit pAb (AP1513)

    Western blot analysis of lysates from A-431 cells using Pan Phospho-T Rabbit pAb (AP1513) at 1:900 dilution. A-431 cells were treated by Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - Pan Phospho-T Rabbit pAb (AP1513)}

    Western blot - Pan Phospho-T Rabbit pAb (AP1513)

    Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-T Rabbit pAb (AP1513) at 1:900 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.