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Pan Phospho-Tyrosine Mouse mAb (AP0973)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Pan Phospho-Tyrosine Mouse mAb (AP0973)

Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine Mouse mAb (AP0973) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Pan Phospho-Tyrosine Mouse mAb (AP0973)

Immunofluorescence analysis of NIH-3T3 cells using Pan Phospho-Tyrosine Mouse mAb (AP0973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePan Phospho-Tyrosine Mouse mAb
Catalog No.AP0973
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG2b, kappa
CloneNo.AMC0235
Protein phosphorylation is one of the main key regulatory mechanisms by which extracellular signals are conveyed. Tyrosine kinase activation is impactful in many tumor types, and in fact, tyrosine kinase inhibitors are one of the more useful therapeutics currently in cancer treatment.
ImmunogenA synthetic peptide corresponding to a sequence containing phosphorylated Y.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat treated by Pervanadate
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Pan Phospho-Tyrosine Mouse mAb (AP0973)}

    Western blot - Pan Phospho-Tyrosine Mouse mAb (AP0973)

    Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine Mouse mAb (AP0973) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - Pan Phospho-Tyrosine Mouse mAb (AP0973)}

    Immunofluorescence - Pan Phospho-Tyrosine Mouse mAb (AP0973)

    Immunofluorescence analysis of NIH-3T3 cells using Pan Phospho-Tyrosine Mouse mAb (AP0973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.