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Pan Phospho-Tyrosine Rabbit mAb (AP1162)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Pan Phospho-Tyrosine Rabbit mAb (AP1162)

Western blot analysis of various lysates using Pan Phospho-Tyrosine Rabbit mAb (AP1162) at 1:1000 dilution. Jurkat cells and NIH/3T3 cells and C6 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunoprecipitation - Pan Phospho-Tyrosine Rabbit mAb (AP1162)

Immunoprecipitation analysis of 300 μg extracts of Jurkat (pervanadate) cells using 3 μg Pan Phospho-Tyrosine antibody (AP1162). Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody (AP1162) at a dilution of 1:1000.

Overview

Product namePan Phospho-Tyrosine Rabbit mAb
Catalog No.AP1162
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC54383
Tyrosine phosphorylation (pTyr), much of which occurred on localized multiple sites, initiates cellular signaling, governs cellular functions, and its dysregulation is implicated in many diseases, especially cancers. pTyr-specific sensing is of great significance for understanding disease states and developing targeted anticancer drugs.
ImmunogenA random peptide library phosphorylated peptide around Tyr.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesJurkat treated by Pervanadate, NIH/3T3 treated by Pervanadate, C6 treated by Pervanadate
Cellular location
Customer validation

IP(Homo sapiens)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Pan Phospho-Tyrosine Rabbit mAb (AP1162)}

    Western blot - Pan Phospho-Tyrosine Rabbit mAb (AP1162)

    Western blot analysis of various lysates using Pan Phospho-Tyrosine Rabbit mAb (AP1162) at 1:1000 dilution. Jurkat cells and NIH/3T3 cells and C6 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunoprecipitation - Pan Phospho-Tyrosine Rabbit mAb (AP1162)}

    Immunoprecipitation - Pan Phospho-Tyrosine Rabbit mAb (AP1162)

    Immunoprecipitation analysis of 300 μg extracts of Jurkat (pervanadate) cells using 3 μg Pan Phospho-Tyrosine antibody (AP1162). Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody (AP1162) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP1162? Please let us know so that we can cite the reference in this datasheet.