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Pan Phospho-Tyrosine Rabbit mAb (AP1316)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Pan Phospho-Tyrosine Rabbit mAb (AP1316)

Western blot analysis of various lysates, using Pan Phospho-Tyrosine Rabbit mAb (AP1316) at 1:2000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Pan Phospho-Tyrosine Rabbit mAb (AP1316)

Western blot analysis of lysates from NIH/3T3 cells, using Pan Phospho-Tyrosine Rabbit mAb (AP1316) at 1:2000 dilution. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunoprecipitation - Pan Phospho-Tyrosine Rabbit mAb (AP1316)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg Pan Phospho-Tyrosine antibody (AP1316). Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody (AP1316) at a dilution of 1:5000.

Overview

Product namePan Phospho-Tyrosine Rabbit mAb
Catalog No.AP1316
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC54380
Tyrosine phosphorylation (pTyr), much of which occurred on localized multiple sites, initiates cellular signaling, governs cellular functions, and its dysregulation is implicated in many diseases, especially cancers. pTyr-specific sensing is of great significance for understanding disease states and developing targeted anticancer drugs.
ImmunogenA synthetic peptide corresponding to a sequence containing phosphorylated Y.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW>10KDa
Observed MW10-250kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesJurkat treated by Pervanadate, NIH/3T3 treated by Pervanadate
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Pan Phospho-Tyrosine Rabbit mAb (AP1316)}

    Western blot - Pan Phospho-Tyrosine Rabbit mAb (AP1316)

    Western blot analysis of various lysates, using Pan Phospho-Tyrosine Rabbit mAb (AP1316) at 1:2000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - Pan Phospho-Tyrosine Rabbit mAb (AP1316)}

    Western blot - Pan Phospho-Tyrosine Rabbit mAb (AP1316)

    Western blot analysis of lysates from NIH/3T3 cells, using Pan Phospho-Tyrosine Rabbit mAb (AP1316) at 1:2000 dilution. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunoprecipitation - Pan Phospho-Tyrosine Rabbit mAb (AP1316)}

    Immunoprecipitation - Pan Phospho-Tyrosine Rabbit mAb (AP1316)

    Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg Pan Phospho-Tyrosine antibody (AP1316). Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody (AP1316) at a dilution of 1:5000.

    * For research use only. Not for therapeutic or diagnostic purposes.