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Pan Phospho-Tyrosine Rabbit pAb (AP0905)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Pan Phospho-Tyrosine Rabbit pAb (AP0905)

Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine pAb (AP0905) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Pan Phospho-Tyrosine Rabbit pAb (AP0905)

Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine (AP0905) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.C6 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.

ABclonal:Immunofluorescence - Pan Phospho-Tyrosine Rabbit pAb (AP0905)

Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine (AP0905) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.

Overview

Product namePan Phospho-Tyrosine Rabbit pAb
Catalog No.AP0905
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Tyrosine phosphorylation (pTyr), much of which occurred on localized multiple sites, initiates cellular signaling, governs cellular functions, and its dysregulation is implicated in many diseases, especially cancers. pTyr-specific sensing is of great significance for understanding disease states and developing targeted anticancer drugs.
ImmunogenA synthetic peptide corresponding to a sequence containing phosphorylated S & T.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW20-70kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat treated by Pervanadate
Cellular location
Customer validation

WB(Homo sapiens, Mus musculus, Escherichia coli)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Pan Phospho-Tyrosine Rabbit pAb (AP0905)}

    Western blot - Pan Phospho-Tyrosine Rabbit pAb (AP0905)

    Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine pAb (AP0905) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - Pan Phospho-Tyrosine Rabbit pAb (AP0905)}

    Immunofluorescence - Pan Phospho-Tyrosine Rabbit pAb (AP0905)

    Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine (AP0905) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.C6 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.
    ABclonal:Immunofluorescence - Pan Phospho-Tyrosine Rabbit pAb (AP0905)}

    Immunofluorescence - Pan Phospho-Tyrosine Rabbit pAb (AP0905)

    Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine (AP0905) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.

    * For research use only. Not for therapeutic or diagnostic purposes.

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