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Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 Rabbit pAb (AP1256)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 Rabbit pAb (AP1256)

Western blot analysis of lysates from C6 cells, using Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 Rabbit pAb (AP1256) at 1:500 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product namePhospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 Rabbit pAb
Catalog No.AP1256
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Mapk14 (phosphoT180/Y182) monoclonal antibody is provided, clone number M139 is a monoclonal antibody prepared from mice, its clone number is M139. Mapk14 is also known as CSBP, CSPB1, Csbp1, Csbp2, Exip, Hog, MGC105413, Mxi2, Prkm14, Prkm15, RK, Sapk2A, p38, p38Hog, p38alpha; The full name of its gene is Mitogen Activated Protein Kinase 14.
ImmunogenA synthetic phosphorylated peptide around T180 & Y182 of human MAPK14MAPK11 (NP_002742.3).
SequenceMTGYV
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW43kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesC6 treated by UV
Cellular locationcytoplasm, cytosol, nucleoplasm, nucleus.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 Rabbit pAb (AP1256)}

    Western blot - Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 Rabbit pAb (AP1256)

    Western blot analysis of lysates from C6 cells, using Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 Rabbit pAb (AP1256) at 1:500 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.