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Phospho-(Ser/Thr) ATM/ATR Substrate Rabbit pAb (AP0933)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-(Ser/Thr) ATM/ATR Substrate Rabbit pAb (AP0933)

Western blot analysis of various lysates using Phospho-(Ser/Thr) ATM/ATR Substrate pAb (AP0933) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product namePhospho-(Ser/Thr) ATM/ATR Substrate Rabbit pAb
Catalog No.AP0933
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The functionally related ATM (ataxia telangiectasia-mutated) and ATR (ATM-Rad3-related) protein kinases are critical regulators of DNA damage responses in mammalian cells. ATM and ATR share highly overlapping substrate specificities and show a strong preference for the phosphorylation of Serine (S) or Threonine (T) residues followed by Gln. It also called SQ or TQ consensus sites.
ImmunogenA synthetic peptide corresponding to a sequence containing phosphorylated S & T.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW38-68kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat treated by Calyculin A, NIH/3T3 treated by Calyculin A, C6 treated by ATP
Cellular location
Customer validation

WB(Macaca mulatta, Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - Phospho-(Ser/Thr) ATM/ATR Substrate Rabbit pAb (AP0933)}

    Western blot - Phospho-(Ser/Thr) ATM/ATR Substrate Rabbit pAb (AP0933)

    Western blot analysis of various lysates using Phospho-(Ser/Thr) ATM/ATR Substrate pAb (AP0933) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP0933? Please let us know so that we can cite the reference in this datasheet.