제품 > 항체 > 다중클론항체(pAb)

Proteinase K Rabbit pAb (A20453)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Western blot - Proteinase K Rabbit pAb (A20453)

Western blot analysis of recombinant Proteinase K Protein using Proteinase K Rabbit pAb (A20453) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: .

Overview

Product nameProteinase K Rabbit pAb
Catalog No.A20453
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Proteinase K is an endopeptidase belonging to the subtilisin group of serine proteases. Proteinase K was isolated from the mold (Parengyodontium album or previously known as Tritirachium album). Proteinase K has five cysteines. Four of the cysteine residues form two disulfide bonds (34-124 and 179-248, respectively) and one lies below one of the catalytic triads. Proteinase K often binds two calcium ions required for full enzymatic activity. In molecular biology laboratories, proteinase K is useful during preparation of DNA or RNA samples by degrading and inactivating proteins.
ImmunogenRecombinant protein of Proteinase K.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW60kDa
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesProteinase K
Cellular location

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Proteinase K Rabbit pAb (A20453)}

    Western blot - Proteinase K Rabbit pAb (A20453)

    Western blot analysis of recombinant Proteinase K Protein using Proteinase K Rabbit pAb (A20453) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: .

    * For research use only. Not for therapeutic or diagnostic purposes.