제품 > 항체 > 다중클론항체(pAb)

RGL3 Rabbit pAb (A22552)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Arabidopsis thaliana

ABclonal:Western blot - RGL3 Rabbit pAb (A22552)

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using RGL3 Rabbit pAb (A22552) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameRGL3 Rabbit pAb
Catalog No.A22552
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Encodes a DELLA subfamily member that acts as a negative regulator of GA signaling and as a coactivator of ABI3 to promote seed storage protein biosynthesis during the seed maturation stage. Involved in hyperosmotic salinity response, jasmonic acid mediated signaling pathway, negative regulation of gibberellic acid mediated signaling pathway, negative regulation of seed germination, regulation of DNA-templated transcription, regulation of reactive oxygen species metabolic process, regulation of seed dormancy process, response to abscisic acid, response to ethylene, salicylic acid mediated signaling pathway.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-106 of Arabidopsis thaliana RGL3. (NP_197251.1).
SequenceMKRSHQETSVEEEAPSMVEKLENGCGGGGDDNMDEFLAVLGYKVRSSDMADVAQKLEQLEMVLSNDIASSSNAFNDTVHYNPSDLSGWAQSMLSDLNYYPDLDPNR
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW62kDa
ReactivityArabidopsis thaliana
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSeedling, Inflorescences, Before inflorescence, Rosette leaf
Cellular location

Documents

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    ABclonal:Western blot - RGL3 Rabbit pAb (A22552)}

    Western blot - RGL3 Rabbit pAb (A22552)

    Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using RGL3 Rabbit pAb (A22552) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.