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Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Goat

ABclonal:Western blot - Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643)

Western blot analysis of recombinant Goat IgG Fab fragment using Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643) at 1:1900 dilution.
Lysates/proteins: 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Flow CytoMetry - Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643)

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and A-431 cells (right) were surface-stained with Human TROP-2 Antibody (2 μg/mL, orange line) or Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643, 5 μl/Test, blue line), follow by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line). Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643, 5 μl/Test) was used as a secondary antibody.

Overview

Product nameRabbit Anti-Goat IgG (Fab) fragment mAb
Catalog No.A25643
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57078
ImmunogenRecombinant protein of Goat IgG (Fab) fragment.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa
ReactivityGoat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • FC 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Flow Cytometry    
Positive samplesRecombinant Goat IgG Fab fragment
Cellular location

Documents

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Lot number

    ABclonal:Western blot - Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643)}

    Western blot - Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643)

    Western blot analysis of recombinant Goat IgG Fab fragment using Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643) at 1:1900 dilution.
    Lysates/proteins: 100ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Flow CytoMetry - Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643)}

    Flow CytoMetry - Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643)

    Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and A-431 cells (right) were surface-stained with Human TROP-2 Antibody (2 μg/mL, orange line) or Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643, 5 μl/Test, blue line), follow by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line). Rabbit Anti-Goat IgG (Fab) fragment mAb (A25643, 5 μl/Test) was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.