Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rabbit IgG isotype control (AC042) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Rabbit IgG isotype control (AC042) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using Rabbit IgG isotype control (AC042) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Confocal imaging of HeLa cells using Rabbit IgG isotype control (AC042, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells using Rabbit IgG isotype control (AC042, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of PC-12 cells using Rabbit IgG isotype control (AC042, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from HeLa cells transfected with a RXRB expression vector containing a single C-terminal flag-Tag, using 3 μg of Rabbit IgG isotype control (AC042) and RXRB Rabbit mAb (A25648). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.
Flow cytometry: Daudi cells were stained with Rabbit IgG isotype control(AC042, 10 μg/mL, blue line) followed by goat anti-Rabbit pAb APC(1:600 dilution) staining. Non-fluorescently stained Daudi cells was used as blank control (red line).
| Product name | Rabbit IgG isotype control |
|---|---|
| Catalog No. | AC042 |
| Host species | Rabbit |
| Purification method | Protein A/G purification |
| Isotype | IgG |
| CloneNo. | ARC5105-03 |
| Immunogen | None |
|---|---|
| Sequence | Email for sequence |
| Gene ID | |
| Swiss Prot | |
| Synonyms | |
| Calculated MW | |
| Observed MW |
| Reactivity | |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Immunohistochemistry Immunofluorescence Immunoprecipitation Flow Cytometry |
| Positive samples | HepG2 |
| Cellular location | |
| Customer validation | FC(Homo sapiens) RIP(Rattus norvegicus) ChIP(Mus musculus) IF(Mus musculus) WB(Mus musculus, Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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