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Rabbit anti GFP-Tag mAb (AE078)

Publications (11) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal: - Rabbit anti GFP-Tag mAb (AE078)

Chromatin immunoprecipitation was performed with cross-linked chromatin from Arabidopsis thaliana leaf cells transfered with GFP, using Rabbit anti GFP-Tag mAb (AE078) and Rabbit IgG (AC042).The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.

ABclonal:Western blot - Rabbit anti GFP-Tag mAb (AE078)

Western blot analysis of lysates from wild type (WT) and insect cells transfected with GFP using Rabbit anti GFP-Tag mAb (AE078) at 1:200000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 30s.

ABclonal:Western blot - Rabbit anti GFP-Tag mAb (AE078)

Western blot analysis of lysates from insect cells transfected with GFP using Rabbit anti GFP-Tag mAb (AE078) at 1:10000-1:2560000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 3s.

ABclonal:Immunofluorescence - Rabbit anti GFP-Tag mAb (AE078)

Immunofluorescence analysis of 293T and 293T-GFP cells using Rabbit anti GFP-Tag mAb (AE078) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Rabbit anti GFP-Tag mAb (AE078)

Immunoprecipitation of SERPINB1-GFP from 150 µg extracts of 293F cells transfected with a SERPINB1 expression vector containing a single N-terminal GFP-Tag was performed using 3 µg of Rabbit anti GFP-Tag mAb (AE078). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. The IP sample was eluted with 1X reducing Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Mouse anti GFP-Tag mAb ( AE012) at a dilution of 1:5000.

Overview

Product nameRabbit anti GFP-Tag mAb
Catalog No.AE078
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC50809
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-238 of GFP.
SequenceMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
Gene ID
Swiss Prot
SynonymsGFP; GFP tag; GFP-tag
Calculated MW27kDa
Observed MW27kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:50000 - 1:700000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 100μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • CHIP 5μg antibody for 10μg-15μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesinsect
Cellular location
Customer validation

IP(Homo sapiens , Mus musculus)

WB(Other, Homo sapiens, Mus musculus)

IF(Homo sapiens)

IP(Other)

IHC(Mus musculus)

Co-IP(Oryza sativa, Other)

Documents

Certificate of Compliance

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Lot number

    ABclonal: - Rabbit anti GFP-Tag mAb (AE078)}

    - Rabbit anti GFP-Tag mAb (AE078)

    Chromatin immunoprecipitation was performed with cross-linked chromatin from Arabidopsis thaliana leaf cells transfered with GFP, using Rabbit anti GFP-Tag mAb (AE078) and Rabbit IgG (AC042).The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.
    ABclonal:Western blot - Rabbit anti GFP-Tag mAb (AE078)}

    Western blot - Rabbit anti GFP-Tag mAb (AE078)

    Western blot analysis of lysates from wild type (WT) and insect cells transfected with GFP using Rabbit anti GFP-Tag mAb (AE078) at 1:200000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 20 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020)
    .Exposure time: 30s.
    ABclonal:Western blot - Rabbit anti GFP-Tag mAb (AE078)}

    Western blot - Rabbit anti GFP-Tag mAb (AE078)

    Western blot analysis of lysates from insect cells transfected with GFP using Rabbit anti GFP-Tag mAb (AE078) at 1:10000-1:2560000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 20 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020)
    .Exposure time: 3s.
    ABclonal:Immunofluorescence - Rabbit anti GFP-Tag mAb (AE078)}

    Immunofluorescence - Rabbit anti GFP-Tag mAb (AE078)

    Immunofluorescence analysis of 293T and 293T-GFP cells using Rabbit anti GFP-Tag mAb (AE078) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - Rabbit anti GFP-Tag mAb (AE078)}

    Immunoprecipitation - Rabbit anti GFP-Tag mAb (AE078)

    Immunoprecipitation of SERPINB1-GFP from 150 µg extracts of 293F cells transfected with a SERPINB1 expression vector containing a single N-terminal GFP-Tag was performed using 3 µg of Rabbit anti GFP-Tag mAb (AE078). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. The IP sample was eluted with 1X reducing Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Mouse anti GFP-Tag mAb ( AE012) at a dilution of 1:5000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE078? Please let us know so that we can cite the reference in this datasheet.