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Rabbit anti GST-Tag pAb (AE006)

Publications (34) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Rabbit anti GST-Tag pAb
ABclonal:Western blot - Rabbit anti GST-Tag pAb (AE006)

Western blot analysis of over-expressed GST protein using Rabbit anti GST-Tag pAb (AE006) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Rabbit anti GST-Tag pAb (AE006)

Western blot analysis of over-expressed GST protein using Rabbit anti GST-Tag pAb (AE006) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Rabbit anti GST-Tag pAb (AE006)

Immunoprecipitation analysis of 50 μg extracts of GST Protein using 3 μg GST-Tag antibody (AE006). Western blot was performed from the immunoprecipitate using GST-Tag antibody (AE006).

Overview

Product nameRabbit anti GST-Tag pAb
Catalog No.AE006
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
ImmunogenRecombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein.
SequenceMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
Gene ID
Swiss Prot
SynonymsGST; GST tag; GST-tag
Calculated MW26kDa
Observed MW27kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IP 3μg antibody for 50μg extracts of recombinant protein
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesGST, GST fusion protein
Cellular location
Customer validation

WB(Homo sapiens, Dendroaspis polylepis, Rattus norvegicus, Mus musculus, Solanum tuberosum , Arabidopsis thaliana, Mus musculus, Zea mays, Other, Nicotiana benthamiana, Saccharomyces cerevisiae)

IP(Homo sapiens, Homo sapiens )

Co-IP(Homo sapiens, Mus musculus)

Other(Arabidopsis thaliana)

IF(Mus musculus)

IP(Mus musculus)

Co-IP(Gallus gallus)

Pull-down(Escherichia coli, Arabidopsis thaliana, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Rabbit anti GST-Tag pAb (AE006)}

    Western blot - Rabbit anti GST-Tag pAb (AE006)

    Western blot analysis of over-expressed GST protein using Rabbit anti GST-Tag pAb (AE006) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - Rabbit anti GST-Tag pAb (AE006)}

    Western blot - Rabbit anti GST-Tag pAb (AE006)

    Western blot analysis of over-expressed GST protein using Rabbit anti GST-Tag pAb (AE006) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunoprecipitation - Rabbit anti GST-Tag pAb (AE006)}

    Immunoprecipitation - Rabbit anti GST-Tag pAb (AE006)

    Immunoprecipitation analysis of 50 μg extracts of GST Protein using 3 μg GST-Tag antibody (AE006). Western blot was performed from the immunoprecipitate using GST-Tag antibody (AE006).

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE006? Please let us know so that we can cite the reference in this datasheet.