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Rabbit anti HA-Tag pAb (AE036)

Publications (35) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Rabbit anti HA-Tag pAb (AE036)

Western blot analysis of lysates from 293T cells, using Rabbit anti HA-Tag pAb (AE036) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - Rabbit anti HA-Tag pAb (AE036)

Immunofluorescence analysis of 293T-HA-C and 293T-HA-N cells using Rabbit anti HA-Tag pAb (AE036) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRabbit anti HA-Tag pAb
Catalog No.AE036
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to HA-Tag.
SequenceYPVDVPDYA
Gene ID
Swiss Prot
SynonymsHA; HA tag; HA-tag
Calculated MW
Observed MW26kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:6000
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T
Cellular location
Customer validation

WB(Homo sapiens, Mus musculus, Arabidopsis thaliana, Penicillium oxalicum, Chlamydomonas, S. cerevisiae, Cyprinus Carpio, Other)

IP(Homo sapiens, Mus musculus)

IF(Mus musculus)

IF(Homo sapiens)

WB(Mus musculus)

IP(Mus musculus, Homo sapiens)

Co-IP(Homo sapiens)

Co-IP(Oryza sativa, Arabidopsis thaliana)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Rabbit anti HA-Tag pAb (AE036)}

    Western blot - Rabbit anti HA-Tag pAb (AE036)

    Western blot analysis of lysates from 293T cells, using Rabbit anti HA-Tag pAb (AE036) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunofluorescence - Rabbit anti HA-Tag pAb (AE036)}

    Immunofluorescence - Rabbit anti HA-Tag pAb (AE036)

    Immunofluorescence analysis of 293T-HA-C and 293T-HA-N cells using Rabbit anti HA-Tag pAb (AE036) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE036? Please let us know so that we can cite the reference in this datasheet.