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Rabbit anti His-Tag pAb (AE068)

Publications (17) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Rabbit anti His-Tag pAb (AE068)

Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Rabbit anti His-Tag pAb (AE068) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Rabbit anti His-Tag pAb (AE068)

Immunofluorescence analysis of HeLa cells using Rabbit anti His-Tag pAb (AE068) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)

Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-Tag with 3 μg Rabbit anti His-Tag pAb antibody（AE068).Western blot was performed from the immunoprecipitate using Rabbit anti His-Tag pAb antibody（AE068) at 1:5000 dilution.

ABclonal:Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)

Immunoprecipitation analysis of extracts of cells using His-Tag pAb (AE068). Western blot was performed from the immunoprecipitate using His-Tag pAb (AE068) at a dilition of 1:3000.

Overview

Product nameRabbit anti His-Tag pAb
Catalog No.AE068
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
ImmunogenA synthetic peptide corresponding to His tag.
SequenceHHHHHH
Gene ID
Swiss Prot
SynonymsHis; His tag; His-tag
Calculated MW
Observed MW35kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular location
Customer validation

IF(Homo sapiens, Sus scrofa, Other)

WB(Homo sapiens, Pseudosciaena crocea, Danio rerio, Mus musculus, Chlorocebus aethiops)

IF(Danio rerio)

Other(Sus scrofa)

Co-IP(Homo sapiens)

ELISA(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Rabbit anti His-Tag pAb (AE068)}

    Western blot - Rabbit anti His-Tag pAb (AE068)

    Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Rabbit anti His-Tag pAb (AE068) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - Rabbit anti His-Tag pAb (AE068)}

    Immunofluorescence - Rabbit anti His-Tag pAb (AE068)

    Immunofluorescence analysis of HeLa cells using Rabbit anti His-Tag pAb (AE068) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)}

    Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)

    Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-Tag with 3 μg Rabbit anti His-Tag pAb antibody（AE068).Western blot was performed from the immunoprecipitate using Rabbit anti His-Tag pAb antibody（AE068) at 1:5000 dilution.
    ABclonal:Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)}

    Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)

    Immunoprecipitation analysis of extracts of cells using His-Tag pAb (AE068). Western blot was performed from the immunoprecipitate using His-Tag pAb (AE068) at a dilition of 1:3000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE068? Please let us know so that we can cite the reference in this datasheet.