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Rabbit anti His-Tag pAb (AE068)

Publications (15) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Rabbit anti His-Tag pAb (AE068)

Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Rabbit anti His-Tag pAb (AE068) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Rabbit anti His-Tag pAb (AE068)

Immunofluorescence analysis of HeLa cells using Rabbit anti His-Tag pAb (AE068) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)

Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-Tag with 3 μg Rabbit anti His-Tag pAb antibody（AE068).Western blot was performed from the immunoprecipitate using Rabbit anti His-Tag pAb antibody（AE068) at 1:5000 dilution.

ABclonal:Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)

Immunoprecipitation analysis of extracts of cells using His-Tag pAb (AE068). Western blot was performed from the immunoprecipitate using His-Tag pAb (AE068) at a dilition of 1:3000.

Overview

Product nameRabbit anti His-Tag pAb
Catalog No.AE068
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
ImmunogenA synthetic peptide corresponding to His tag.
SequenceHHHHHH
Gene ID
Swiss Prot
SynonymsHis; His tag; His-tag
Calculated MW
Observed MW35kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular location
Customer validation

IF(Homo sapiens, Sus scrofa, Other)

WB(Homo sapiens, Pseudosciaena crocea, Danio rerio, Mus musculus)

IF(Danio rerio)

Other(Sus scrofa)

Co-IP(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Rabbit anti His-Tag pAb (AE068)}

    Western blot - Rabbit anti His-Tag pAb (AE068)

    Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Rabbit anti His-Tag pAb (AE068) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - Rabbit anti His-Tag pAb (AE068)}

    Immunofluorescence - Rabbit anti His-Tag pAb (AE068)

    Immunofluorescence analysis of HeLa cells using Rabbit anti His-Tag pAb (AE068) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)}

    Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)

    Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-Tag with 3 μg Rabbit anti His-Tag pAb antibody（AE068).Western blot was performed from the immunoprecipitate using Rabbit anti His-Tag pAb antibody（AE068) at 1:5000 dilution.
    ABclonal:Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)}

    Immunoprecipitation - Rabbit anti His-Tag pAb (AE068)

    Immunoprecipitation analysis of extracts of cells using His-Tag pAb (AE068). Western blot was performed from the immunoprecipitate using His-Tag pAb (AE068) at a dilition of 1:3000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE068? Please let us know so that we can cite the reference in this datasheet.