제품 > 항체 > Tag & 로딩 컨트롤 항체

Rabbit anti His-tag mAb (AE086)

Publications (11) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Rabbit anti His-tag mAb (AE086)

Western blot analysis of lysates from His Protein, using Rabbit anti His-tag mAb (AE086) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Rabbit anti His-tag mAb (AE086)

Immunofluorescence analysis of 293T-SOST His(C) and 293T cells using Rabbit anti His-tag mAb (AE086) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Rabbit anti His-tag mAb (AE086)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg His-tag antibody (AE086). Western blot was performed from the immunoprecipitate using His-tag antibody (AE086) at a dilution of 1:30000.

Overview

Product nameRabbit anti His-tag mAb
Catalog No.AE086
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2696
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
ImmunogenA synthetic peptide corresponding to His tag.The antibody may cross-react with a protein of unknown origin ~60kDa
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsHis; His tag; His-tag
Calculated MW
Observed MW16kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:40000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHis fusion protein
Cellular location
Customer validation

WB(Escherichia coli, Mus musculus, Homo sapiens, Mus musculus, Oryza sativa, Other, Zea mays)

IP(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

Pull-down(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Rabbit anti His-tag mAb (AE086)}

    Western blot - Rabbit anti His-tag mAb (AE086)

    Western blot analysis of lysates from His Protein, using Rabbit anti His-tag mAb (AE086) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - Rabbit anti His-tag mAb (AE086)}

    Immunofluorescence - Rabbit anti His-tag mAb (AE086)

    Immunofluorescence analysis of 293T-SOST His(C) and 293T cells using Rabbit anti His-tag mAb (AE086) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - Rabbit anti His-tag mAb (AE086)}

    Immunoprecipitation - Rabbit anti His-tag mAb (AE086)

    Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg His-tag antibody (AE086). Western blot was performed from the immunoprecipitate using His-tag antibody (AE086) at a dilution of 1:30000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE086? Please let us know so that we can cite the reference in this datasheet.