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Rabbit anti-Monkey IgE mAb (A27383)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Cynomolgus monkey

ABclonal:Flow CytoMetry - Rabbit anti-Monkey IgE mAb (A27383)

Flow cytometry: 1X10^6 Monkey PBMC were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or Rabbit anti-Monkey IgE mAb (A27383, 2 μg/mL, right), followed by Alexa Fluor® 488 conjugated goat anti-rabbit pAb staining.

Overview

Product nameRabbit anti-Monkey IgE mAb
Catalog No.A27383
Host speciesRabbit
Purification method
IsotypeIgG
CloneNo.ARC61158
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 99-429 of Monkey IgE(EHH62331.1).
SequenceNKTFGVCSRNFTPPTVKILQSSCDDDGHFPPTIQLLCLISGYTPGAINVTWLENGQVMKVNSPTPPATQEGELASTQSEFTLAQKHWLSDRTYTCQVTYQGTTYNDSTKKCADSNPRGVSAYLSRPSPFDLFISKSPTITCLVVDLAPSKETVNLTWSRASGKPVPHIPATEKKQQRNGTLTVTSILPVVTQDWIEGETYQCRVIHPHLPRALVRSMTKTSGPRAAPEVYVFATPEKLESRDKRTLACLIENFMPEDISVQWLHSDVQLPDTRHSVTQPRKTKGSGFFVFSRLEVTKAEWEQKDEFICRAVHEAASPSWIVQQAVSVNPGK
Gene ID
Swiss Prot
SynonymsIgE; IGHE
Calculated MW
Observed MW
ReactivityCynomolgus monkey
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - Rabbit anti-Monkey IgE mAb (A27383)}

    Flow CytoMetry - Rabbit anti-Monkey IgE mAb (A27383)

    Flow cytometry: 1X10^6 Monkey PBMC were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or Rabbit anti-Monkey IgE mAb (A27383, 2 μg/mL, right), followed by Alexa Fluor® 488 conjugated goat anti-rabbit pAb staining.

    * For research use only. Not for therapeutic or diagnostic purposes.