| Product name | Rabbit anti-Mouse G-CSF mAb (DET) |
|---|---|
| Catalog No. | RMK0112 |
| Catagory | Elisa Antibody Kit |
| Application | ELISA |
| Description | Mouse granulocyte-colony stimulating factor (G-CSF) is a 24-25 kDa monomeric glycoprotein that regulates the proliferation, differentiation and activation of hematopoietic cells. Mouse G-CSF cDNA encodes a 208 amino acid (aa) precursor protein with a 30 aa signal sequence that is proteolytically cleaved to form a 178 aa O-glycosylated mature protein containing two intrachain disulfide bridges. In humans, two distinct cDNA clones, encoding a 204 aa form and a minor alternatively spliced 207 aa form of G-CSF precursors, have been isolated.Mouse and human G-CSF are 76% identical at the aa sequence level and the two proteins show species cross-reactivity. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-α or IL-1. Other cell types, including fibroblasts, endothelial cells, astrocytes and bone marrow stroma cells, can also secrete G-CSF after activation. In addition, various tumor cells express G-CSF constitutively. |
| Immunogen | Recombinant Mouse G-CSF |
| Purirty | Affinity purification |
| Ig Type | Rabbit IgG |
| Formulation | Supplied as a 0.2um filtered solution in PBS with 0.05%ProClin 300, PH 7.4. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Preservative：0.05%ProClin 300. Avoid repeated freeze-thaw cycles. |
Mouse G-CSF Sandwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 1-4ug/mL | Rabbit anti-Mouse G-CSF (CAP)(Cat. No.RMK0111) |
| ELISA Detection | 0.008-0.033ug/mL | Rabbit anti-Mouse G-CSF (DET)(Cat. No.RMK0112) |
| Standard | 7.8-500pg/mL | Recombinant Mouse G-CSF Protein(Cat. No.RP00573) |
