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Rabbit anti Myc-Tag pAb (AE009)

Publications (19) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Rabbit anti Myc-Tag pAb
ABclonal:Western blot - Rabbit anti Myc-Tag pAb (AE009)

Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Rabbit anti Myc-Tag pAb (AE009) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Rabbit anti Myc-Tag pAb (AE009)

Immunofluorescence analysis of HeLa-Myc-C and HeLa cells using Rabbit anti Myc-Tag pAb (AE009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Rabbit anti Myc-Tag pAb antibody (AE009). Western blot was performed from the immunoprecipitate using Rabbit anti Myc-Tag pAb antibody (AE009) at a dilution of 1:50000.

ABclonal:Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)

Immunoprecipitation analysis of 300 μg extracts of 293T-NLK-Myc cells using 3 μg Myc-Tag antibody (AE009). Western blot was performed from the immunoprecipitate using Myc-Tag antibody (AE009) at a dilution of 1:20000.

Overview

Product nameRabbit anti Myc-Tag pAb
Catalog No.AE009
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to Myc tag.
SequenceEQKLISEEDL
Gene ID
Swiss Prot
SynonymsMyc; Myc tag; Myc-tag
Calculated MW
Observed MW60kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:6000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T-NLK-Myc
Cellular location
Customer validation

WB(Homo sapiens, Gallus gallus, Arabidopsis thaliana, Solanum tuberosum , Oryctolagus cuniculus, Mus musculus, Oryza sativa)

Co-IP(Homo sapiens, Mus musculus, Arabidopsis thaliana)

IHC(Sus scrofa)

Co-IP(Homo sapiens)

IP(Homo sapiens, Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Rabbit anti Myc-Tag pAb (AE009)}

    Western blot - Rabbit anti Myc-Tag pAb (AE009)

    Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Rabbit anti Myc-Tag pAb (AE009) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - Rabbit anti Myc-Tag pAb (AE009)}

    Immunofluorescence - Rabbit anti Myc-Tag pAb (AE009)

    Immunofluorescence analysis of HeLa-Myc-C and HeLa cells using Rabbit anti Myc-Tag pAb (AE009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)}

    Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)

    Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Rabbit anti Myc-Tag pAb antibody (AE009). Western blot was performed from the immunoprecipitate using Rabbit anti Myc-Tag pAb antibody (AE009) at a dilution of 1:50000.
    ABclonal:Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)}

    Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)

    Immunoprecipitation analysis of 300 μg extracts of 293T-NLK-Myc cells using 3 μg Myc-Tag antibody (AE009). Western blot was performed from the immunoprecipitate using Myc-Tag antibody (AE009) at a dilution of 1:20000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE009? Please let us know so that we can cite the reference in this datasheet.