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Rabbit anti V5-Tag mAb (AE101)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Rabbit anti V5-Tag mAb (AE101)

Western blot analysis of extracts of normal 293T cells and 293T transfected with V5 Protein, using Rabbit anti V5-Tag mAb (AE101) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Rabbit anti V5-Tag mAb (AE101)

Western blot analysis of lysates from 293T cells, using Rabbit anti V5-Tag mAb (AE101) at 1:10000-1:320000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunoprecipitation - Rabbit anti V5-Tag mAb (AE101)

Immunoprecipitation analysis of 300 μg extracts of CHO-V5 cells using 3 μg Rabbit anti V5-Tag antibody (AE101). Western blot was performed from the immunoprecipitate using Rabbit anti V5-Tag antibody (AE101) at a dilution of 1:5000.

Overview

Product nameRabbit anti V5-Tag mAb
Catalog No.AE101
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC50435
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to V5-Tag.
SequenceGKPIPNPLLGLDST
Gene ID
Swiss Prot
SynonymsV5; V5 tag; V5-tag
Calculated MW
Observed MWRefer to figures
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:20000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samples
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Rabbit anti V5-Tag mAb (AE101)}

    Western blot - Rabbit anti V5-Tag mAb (AE101)

    Western blot analysis of extracts of normal 293T cells and 293T transfected with V5 Protein, using Rabbit anti V5-Tag mAb (AE101) at 1:20000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - Rabbit anti V5-Tag mAb (AE101)}

    Western blot - Rabbit anti V5-Tag mAb (AE101)

    Western blot analysis of lysates from 293T cells, using Rabbit anti V5-Tag mAb (AE101) at 1:10000-1:320000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunoprecipitation - Rabbit anti V5-Tag mAb (AE101)}

    Immunoprecipitation - Rabbit anti V5-Tag mAb (AE101)

    Immunoprecipitation analysis of 300 μg extracts of CHO-V5 cells using 3 μg Rabbit anti V5-Tag antibody (AE101). Western blot was performed from the immunoprecipitate using Rabbit anti V5-Tag antibody (AE101) at a dilution of 1:5000.

    * For research use only. Not for therapeutic or diagnostic purposes.