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Rabbit anti V5-Tag pAb (AE071)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Rabbit anti V5-Tag pAb (AE071)

Western blot analysis of extracts of normal 293T cells and 293T transfected with V5-Wnt3a3d protein, using Rabbit anti V5-Tag pAb (AE071) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameRabbit anti V5-Tag pAb
Catalog No.AE071
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to V5 tag.
SequenceGKPIPNPLLGLDST
Gene ID
Swiss Prot
SynonymsV5; V5 tag; V5-tag
Calculated MW
Observed MWRefer to Figures
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular location
Customer validation

Co-IP(Other)

Documents

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    ABclonal:Western blot - Rabbit anti V5-Tag pAb (AE071)}

    Western blot - Rabbit anti V5-Tag pAb (AE071)

    Western blot analysis of extracts of normal 293T cells and 293T transfected with V5-Wnt3a3d protein, using Rabbit anti V5-Tag pAb (AE071) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE071? Please let us know so that we can cite the reference in this datasheet.