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Rabbit anti mCherry-Tag mAb (AE127)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Rabbit anti mCherry-Tag mAb (AE127)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with mCherry using Rabbit anti mCherry-Tag mAb (AE127) at 1:20000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 45s.

ABclonal:Immunoprecipitation - Rabbit anti mCherry-Tag mAb (AE127)

Immunoprecipitation of CT55-mCherry from 300 µg extracts of 293F cells transfected with a CT55 expression vector containing a single C-terminal mCherry-Tag was performed using 3 µg of mCherry-Tag Rabbit mAb (AE127). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using mCherry-Tag Rabbit mAb (AE127) at a dilution of 1:6000.

Overview

Product nameRabbit anti mCherry-Tag mAb
Catalog No.AE127
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-236 of Discosoma MCherry fluorescent proteinm.
SequenceMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
Gene ID
Swiss Prot
SynonymsmCherry; mCherry tag; mCherry-tag
Calculated MW
Observed MW33kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:40000
  • IP 0.5μg-4μg antibody for 200μg-600μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samples293F
Cellular location
Customer validation

WB(Agrobacterium)

Co-IP(Agrobacterium)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Rabbit anti mCherry-Tag mAb (AE127)}

    Western blot - Rabbit anti mCherry-Tag mAb (AE127)

    Western blot analysis of lysates from wild type (WT) and 293F cells transfected with mCherry using Rabbit anti mCherry-Tag mAb (AE127) at 1:20000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 20 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020)
    .Exposure time: 45s.
    ABclonal:Immunoprecipitation - Rabbit anti mCherry-Tag mAb (AE127)}

    Immunoprecipitation - Rabbit anti mCherry-Tag mAb (AE127)

    Immunoprecipitation of CT55-mCherry from 300 µg extracts of 293F cells transfected with a CT55 expression vector containing a single C-terminal mCherry-Tag was performed using 3 µg of mCherry-Tag Rabbit mAb (AE127). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using mCherry-Tag Rabbit mAb (AE127) at a dilution of 1:6000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE127? Please let us know so that we can cite the reference in this datasheet.