제품 > 항체 > 2차 항체

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (AS040)

Publications (30) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Immunofluorescence - Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (AS040)

Immunofluorescence analysis of HeLa cells, using Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:200 dilution.
Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (AS040)

Immunofluorescence analysis of HeLa cells, using Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:100 dilution.
Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (AS040)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test, orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)(AS040, 1:200) was used as a secondary antibody.

Overview

Product nameRhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
Catalog No.AS040
Host speciesGoat
Purification methodAffinity purification
IsotypeTRITC conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • FC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

(Mus musculus)

(Mus musculus)

IF(Rattus norvegicus, Oryctolagus cuniculus, Homo sapiens, Mus musculus, Saccharomyces cerevisiae, Gallus gallus, Mus musculus, Other)

IHC(Mus musculus, Other)

IF(Mus musculus)

WB(Mus musculus, Homo sapiens)

IHC(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Immunofluorescence - Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (AS040)}

    Immunofluorescence - Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (AS040)

    Immunofluorescence analysis of HeLa cells, using Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
    Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:200 dilution.
    Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (AS040)}

    Immunofluorescence - Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (AS040)

    Immunofluorescence analysis of HeLa cells, using Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
    Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:100 dilution.
    Blue: DAPI for nuclear staining.
    ABclonal:Flow CytoMetry - Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (AS040)}

    Flow CytoMetry - Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (AS040)

    Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test, orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)(AS040, 1:200) was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS040? Please let us know so that we can cite the reference in this datasheet.