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Rhodamine-conjugated Goat anti-Rat IgG (H+L) (AS022)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:-

ABclonal:Flow CytoMetry - Rhodamine-conjugated Goat anti-Rat IgG (H+L) (AS022)

Flow cytometry: 1X10^6 RK13 cells (negative control, left) and RK13-CD20 transfection cells (right) were surface-stained with rat anti-mouse CD20 Antibody (1:100, orange line) or secondary antibody only (blue line). Non-fluorescently stained RK13 and RK13 transfection cells were used as blank control (red line). Rhodamine Goat Anti-Rat IgG (H+L)(AS022, 1:200) was used as a secondary antibody.

Overview

Product nameRhodamine-conjugated Goat anti-Rat IgG (H+L)
Catalog No.AS022
Host speciesGoat
Purification methodAffinity purification
IsotypeRhodamine conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRat IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity-
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - Rhodamine-conjugated Goat anti-Rat IgG (H+L) (AS022)}

    Flow CytoMetry - Rhodamine-conjugated Goat anti-Rat IgG (H+L) (AS022)

    Flow cytometry: 1X10^6 RK13 cells (negative control, left) and RK13-CD20 transfection cells (right) were surface-stained with rat anti-mouse CD20 Antibody (1:100, orange line) or secondary antibody only (blue line). Non-fluorescently stained RK13 and RK13 transfection cells were used as blank control (red line). Rhodamine Goat Anti-Rat IgG (H+L)(AS022, 1:200) was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS022? Please let us know so that we can cite the reference in this datasheet.