제품 > 항체 > Tag & 로딩 컨트롤 항체

S-Tag Rabbit pAb (A24465)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - S-Tag Rabbit pAb (A24465)

Western blot analysis of lysates from wild type (WT) and 293T transfected with S-Tag using S-Tag Rabbit pAb (A24465) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameS-Tag Rabbit pAb
Catalog No.A24465
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A.
ImmunogenA synthesized peptide derived from S-Tag.
SequenceKETAAAKFERQHMDS
Gene ID
Swiss Prot
SynonymsS-Tag; S
Calculated MW
Observed MW30kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T transfected with S-Tag
Cellular location

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - S-Tag Rabbit pAb (A24465)}

    Western blot - S-Tag Rabbit pAb (A24465)

    Western blot analysis of lysates from wild type (WT) and 293T transfected with S-Tag using S-Tag Rabbit pAb (A24465) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.