제품 > 항체 > 메틸화 특이적 항체

pan-Mono-MethylR(R*GG)Motif Rabbit pAb (A18297)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - pan-Mono-MethylR(R*GG)Motif Rabbit pAb (A18297)

Western blot analysis of various lysates using pan-Mono-MethylR(R*GG)Motif pAb (A18297) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Dot Blot - pan-Mono-MethylR(R*GG)Motif Rabbit pAb (A18297)

Dot-blot analysis of all sorts of peptides using pan-Mono-MethylR(R*GG)Motif Rabbit pAb (A18297) at 1:1000 dilution.

Overview

Product namepan-Mono-MethylR(R*GG)Motif Rabbit pAb
Catalog No.A18297
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Arginine methylation is a common posttranslational modification that is found on both histone and non-histone proteins. Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). The most prevalent is omega-NG, NG-dimethylarginine. Here, two methyl groups are placed on one of the terminal nitrogen atoms of the guanidino group; this derivative is commonly referred to as asymmetric dimethylarginine (ADMA). Two other derivatives occur at levels of about 20% to 50% that of ADMA. These include the symmetric dimethylated derivative, where one methyl group is placed on each of the terminal guanidino nitrogens and the monomethylated derivative with a single methyl group on the terminal nitrogen atom . These three derivatives are present on a multitude of distinct protein species in the cytoplasm, nucleus, and organelles of mammalian cells. Methylated arginine residues in proteins are often flanked by one or more glycine residues, but there are many exceptions to this general rule.
ImmunogenA synthetic peptide corresponding to a sequence containing monomethylated R.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW16-120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • DB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHCT116, Mouse lung, Mouse liver, Mouse brain, Rat brain
Cellular location

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - pan-Mono-MethylR(R*GG)Motif Rabbit pAb (A18297)}

    Western blot - pan-Mono-MethylR(R*GG)Motif Rabbit pAb (A18297)

    Western blot analysis of various lysates using pan-Mono-MethylR(R*GG)Motif pAb (A18297) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Dot Blot - pan-Mono-MethylR(R*GG)Motif Rabbit pAb (A18297)}

    Dot Blot - pan-Mono-MethylR(R*GG)Motif Rabbit pAb (A18297)

    Dot-blot analysis of all sorts of peptides using pan-Mono-MethylR(R*GG)Motif Rabbit pAb (A18297) at 1:1000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.