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pan-Nitrific-Y Rabbit pAb (A20441)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - pan-Nitrific-Y Rabbit pAb (A20441)

Western blot analysis of various lysates using pan-Nitrific-Y Rabbit pAb (A20441) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate（3mM）for 1 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - pan-Nitrific-Y Rabbit pAb (A20441)

Western blot analysis of lysates from NIH/3T3 cells, using pan-Nitrific-Y Rabbit pAb (A20441) at 1:1000 dilution.NIH/3T3 cells were treated by Perxynitrate（3mM）for 3 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product namepan-Nitrific-Y Rabbit pAb
Catalog No.A20441
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic peptide corresponding to a sequence containing myristylated K.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW10-250kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa treated by Perxynitrate, C6 treated by Perxynitrate, NIH/3T3 treated by Perxynitrate
Cellular location

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - pan-Nitrific-Y Rabbit pAb (A20441)}

    Western blot - pan-Nitrific-Y Rabbit pAb (A20441)

    Western blot analysis of various lysates using pan-Nitrific-Y Rabbit pAb (A20441) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate（3mM）for 1 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - pan-Nitrific-Y Rabbit pAb (A20441)}

    Western blot - pan-Nitrific-Y Rabbit pAb (A20441)

    Western blot analysis of lysates from NIH/3T3 cells, using pan-Nitrific-Y Rabbit pAb (A20441) at 1:1000 dilution.NIH/3T3 cells were treated by Perxynitrate（3mM）for 3 hours.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.