Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse
Western blot analysis of lysates from NIH/3T3 cells, using pan-Phospho-Serine Rabbit pAb (AP0932) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
| Product name | pan-Phospho-Serine Rabbit pAb |
|---|---|
| Catalog No. | AP0932 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence containing phosphorylated S. |
|---|---|
| Sequence | Email for sequence |
| Gene ID | |
| Swiss Prot | |
| Synonyms | |
| Calculated MW | |
| Observed MW | 60kDa |
| Reactivity | Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | NIH/3T3 treated by Calyculin A |
| Cellular location | |
| Customer validation | WB(Mus musculus, Homo sapiens) |
* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using AP0932? Please let us know so that we can cite the reference in this datasheet.
_WB_01.jpg?t=1718355863 "ABclonal:Western blot - pan-Phospho-Serine Rabbit pAb (AP0932)")