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pan-Symmetric Di-Methyl Arginine Rabbit pAb (A18261)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - pan-Symmetric Di-Methyl Arginine Rabbit pAb (A18261)

Western blot analysis of various lysates using pan-Symmetric-Di-Methyl Arginine Rabbit pAb (A18261) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Dot Blot - pan-Symmetric Di-Methyl Arginine Rabbit pAb (A18261)

Dot-blot analysis of all sorts of methylation peptides using pan Symmetric-DiMethyl-Arginine antibody (A18261) at 1:1000 dilution.

Overview

Product namepan-Symmetric Di-Methyl Arginine Rabbit pAb
Catalog No.A18261
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The methylation of arginine residues in proteins is a post-translational modification that contributes to a wide range of biological processes. Many cytokines involved in T cell development and activation utilize the common cytokine receptor γ-chain (γc) and the kinase JAK3 for signal transduction.
ImmunogenA synthetic peptide corresponding to a sequence containing methylated R.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW22-70kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, MCF7, NIH/3T3, C6, Mouse liver, Rat liver
Cellular location
Customer validation

WB(Mus musculus, C. albicans)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - pan-Symmetric Di-Methyl Arginine Rabbit pAb (A18261)}

    Western blot - pan-Symmetric Di-Methyl Arginine Rabbit pAb (A18261)

    Western blot analysis of various lysates using pan-Symmetric-Di-Methyl Arginine Rabbit pAb (A18261) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Dot Blot - pan-Symmetric Di-Methyl Arginine Rabbit pAb (A18261)}

    Dot Blot - pan-Symmetric Di-Methyl Arginine Rabbit pAb (A18261)

    Dot-blot analysis of all sorts of methylation peptides using pan Symmetric-DiMethyl-Arginine antibody (A18261) at 1:1000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A18261? Please let us know so that we can cite the reference in this datasheet.