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pan-Tri-Methyl lysine Rabbit pAb (A20145)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - pan-Tri-Methyl lysine Rabbit pAb (A20145)

Western blot analysis of various lysates using pan-Tri-Methyl lysine Rabbit pAb (A20145) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunoprecipitation - pan-Tri-Methyl lysine Rabbit pAb (A20145)

Immunoprecipitation analysis of 1.5mg extracts of HeLa cells using 30ug pan-Tri-Methyl lysine antibody (A20145). Western blot was performed from the immunoprecipitate using TriMethyl-Histone H3-K27 antibody (A2363) at a dilution of 1:1000.

ABclonal:Chromatin Immunoprecipitation - pan-Tri-Methyl lysine Rabbit pAb (A20145)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using pan-Tri-Methyl lysine antibody (A20145) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product namepan-Tri-Methyl lysine Rabbit pAb
Catalog No.A20145
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs).The post-translational ε-amino lysine methylated proteins is an important reversible modification which plays a vital role in the regulation of many cellular processes including chromatin dynamics and gene transcription. Methylation of lysine residues is modulated by specific counteractive enzymes including lysine methylases (KMTs) and demethylases (KDMs). Lysine trimethylation occurs in both histones and non-histone substratres. It has become promising targets for discovery of anti-cancer drugs.
ImmunogenA synthetic peptide corresponding to a sequence containing Tri-methylated K.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW15-74kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 3μg antibody for 5μg-10μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesHeLa, MCF7, NIH/3T3, C6
Cellular location
Customer validation

WB(Other)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - pan-Tri-Methyl lysine Rabbit pAb (A20145)}

    Western blot - pan-Tri-Methyl lysine Rabbit pAb (A20145)

    Western blot analysis of various lysates using pan-Tri-Methyl lysine Rabbit pAb (A20145) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunoprecipitation - pan-Tri-Methyl lysine Rabbit pAb (A20145)}

    Immunoprecipitation - pan-Tri-Methyl lysine Rabbit pAb (A20145)

    Immunoprecipitation analysis of 1.5mg extracts of HeLa cells using 30ug pan-Tri-Methyl lysine antibody (A20145). Western blot was performed from the immunoprecipitate using TriMethyl-Histone H3-K27 antibody (A2363) at a dilution of 1:1000.
    ABclonal:Chromatin Immunoprecipitation - pan-Tri-Methyl lysine Rabbit pAb (A20145)}

    Chromatin Immunoprecipitation - pan-Tri-Methyl lysine Rabbit pAb (A20145)

    Chromatin immunoprecipitation analysis of extracts of HeLa cells, using pan-Tri-Methyl lysine antibody (A20145) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20145? Please let us know so that we can cite the reference in this datasheet.