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pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303)

Western blot analysis of lysates from 293T cells using pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303) at 1:1000 dilution. 293T cells were treated by MG132(5 μM) at 37℃ for 4 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303)

Western blot analysis of various lysates using pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product namepan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb
Catalog No.A20303
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ubiquitination plays a key role in protein degradation and signal transduction. Characterization of ubiquitination sites is important for understanding the role of this modification in cellular processes and disease. Ubiquitination sites are usually identified by detection of Lys-?-Gly-Gly (K-?-GG)-remnant peptides, which are generated by tryptic digestion of proteomes. The di-glycine remnant left at sites of ubiquitination after trypsin digestion through cleavage of the C-terminal –RGG sequence on ubiquitin (K-ε-GG). This Ubiquitin K-ε-GG remnant motif antibody will recognize the di-glycine remnant independent of flanking amino acid sequence.
ImmunogenA synthetic peptide corresponding to a sequence containing ubiquitinated Motif (K-ε-GG)
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW>10kDa/＞10kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, NIH/3T3, Mouse brain, C6, Rat heart, 293T treated by MG132
Cellular location

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303)}

    Western blot - pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303)

    Western blot analysis of lysates from 293T cells using pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303) at 1:1000 dilution. 293T cells were treated by MG132(5 μM) at 37℃ for 4 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303)}

    Western blot - pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303)

    Western blot analysis of various lysates using pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.