Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of extracts of various cell lines, using pan-methyl-Histidine antibody (A17948) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 300s.
Dot-blot analysis of all sorts using pan-methyl-Histidine pAb (A17948) at 1:1000 dilution.
Peptide 1：Peptide library (3-methylhistidine- intermediate)
Peptide 2：Peptide library (3-methylhistidine- C-terminal)
Peptide 3：Peptide library (1-methylhistidine- intermediate)
Peptide 4：Peptide library (1-methylhistidine- C-terminal)
Peptide 5：Histidine methylation modified peptide library mix
Peptide 6：Control peptide library mix
| Product name | pan-methyl-Histidine Rabbit pAb |
|---|---|
| Catalog No. | A17948 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide of Methylated Histidine His. |
|---|---|
| Sequence | Email for sequence |
| Gene ID | |
| Swiss Prot | |
| Synonyms | |
| Calculated MW | |
| Observed MW | 10-100kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa, HepG2, A-549, NIH/3T3, C6, Mouse brain, Mouse liver, Rat brain, Rat liver |
| Cellular location | |
| Customer validation | Other(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A17948? Please let us know so that we can cite the reference in this datasheet.
_WB_01.jpg?t=1697792908 "ABclonal:Western blot - pan-methyl-Histidine Rabbit pAb (A17948)")
_DotBlot_01.jpg?t=1697792908 "ABclonal:Dot Blot - pan-methyl-Histidine Rabbit pAb (A17948)")