제품 > 항체 > 다중클론항체(pAb)

plxIS motif Rabbit pAb (A24458)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - plxIS motif Rabbit pAb (A24458)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with plxIS motif using plxIS motif Rabbit pAb (A24458) at 1:400 dilution. 
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameplxIS motif Rabbit pAb
Catalog No.A24458
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthesized peptide derived from plxIS motif.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW＞10kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - plxIS motif Rabbit pAb (A24458)}

    Western blot - plxIS motif Rabbit pAb (A24458)

    Western blot analysis of lysates from wild type (WT) and 293F cells transfected with plxIS motif using plxIS motif Rabbit pAb (A24458) at 1:400 dilution. 
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.