Overview
| Product name | Biotinylated Recombinant Human TNFSF5/CD40 Ligand/CD154 Trimer Protein (Primary Amine Labeling) |
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| Catalog No. | RP00538B |
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| Description | Biotinylated Recombinant Human TNFSF5/CD40 ligand/CD154 Trimer Protein (Primary Amine Labeling) is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met113-Leu261) of Human TNFSF5/CD40 ligand/CD154 Trimer (Primary Amine Labeling) (Accession #P29965) fused with a N-His&Flag tag at the N-terminus. |
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| Purity | > 95% by SDS-PAGE；> 95% by HPLC |
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| Endotoxin | < 1 EU/μg of the protein by LAL method |
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| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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| Species | Human |
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| Calculated MW | 52.4 kDa |
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Background
CD40 ligand or CD40L, also called CD154, ? is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Product information
| Synonym | CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
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| Expression Host | HEK293 cells |
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| Tag | N-His&Flag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
