Overview
| Product name | Recombinant Human TNFSF5/CD40 ligand/CD154 Protein |
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| Catalog No. | RP01733 |
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| Description | Active Recombinant Human CD40-L/CD40L/TNFSF5/TRAP/CD40LG Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly116-Leu261) of human CD40-L/CD40L/TNFSF5/TRAP/CD40LG(Accession #NP_000065.1) fused with and a 6×His tag at the N-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA.Immobilized Human CD40L (Catalog: RP01733) at 5 μg/mL (100 μL/well) can bind Human CD40 (Catalog: RP01218) with a linear range of 0.005-0.5 μg/mL.
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| Endotoxin | < 0.1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 16.64 kDa |
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Background
CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.
Product information
| Synonym | IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L；CD40LG |
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| Expression Host | HEK293 cells |
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| Tag | N-6His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
