Overview
| Product name | Recombinant Human Angiopoietin-like 3/ANGPTL3(17-460) Protein |
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| Catalog No. | RP02866 |
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| Description | Recombinant Human Angiopoietin-like 3/ANGPTL3(17-460) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser17-Glu460) of human Angiopoietin-like 3/ANGPTL3 (Accession #NP_055310.1) fused with a 6×His tag at the C-terminus. |
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| Purity | > 90% as determined by HPLC |
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| Endotoxin | <1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% CHAPS, pH7.4. |
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| Species | Human |
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| Calculated MW | 52.65 kDa |
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Background
Angiopoietin-like protein 3 (ANGPTL3) also known as Angiopoietin-related protein 3, Angiopoietin-5 (ANG-5) is a secreted glycoprotein that is structurally related to the angiopoietins.Human ANGPTL3 contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. The fibrinogen C-terminal domain is sufficient to mediate endothelial cell adhesion. ANGPTL3 is principally expressed in the liver and can exert activities related to both angiogenesis and lipid metabolism.ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids.
Product information
| Synonym | ANG-5; ANGPT5; ANL3; FHBL2; ANGPTL3; ANGPT5; ANL3; FHBL2; ANGPTL3 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
