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Recombinant Human Argonaute-3/AGO3 Protein (RP02965)

Publication (1) Datasheet

Overview

Product nameRecombinant Human Argonaute-3/AGO3 Protein
Catalog No.RP02965
DescriptionRecombinant Human Argonaute-3/AGO3 Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Ala860) of human Argonaute-3/AGO3 (Accession #NP_079128.2) fused with a 6×His tag at the N-terminus.
Purity>88% as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% gly.
SpeciesHuman
Calculated MW99.6 kDa
Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. AGO3 also coexists and interacts with Armitage in the mitochondrial fraction. Furthermore, AGO3 acts in conjunction with the mitochondria-associated protein Zucchini to control the dynamic subcellular localization of Armitage between mitochondria and nuage in a Slicer-dependent fashion.
SynonymEIF2C3; AGO3
Expression HostBaculovirus-Insect Cells
TagN-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using RP02965? Please let us know so that we can cite the reference in this datasheet.

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