Overview
| Product name | Recombinant Human BTN3A1/CD277 Protein |
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| Catalog No. | RP01067 |
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| Description | Recombinant human BTN3A1/CD277 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln30-Gly254) of human BTN3A1/CD277 (Accession #NP_919423.1) fused with an Fc, 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to inhibit Anti-CD3-induced proliferation of jurkat. The ED50 for this effect is 24-96 ng/mL.
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| Purity | > 97% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 50.94 kDa |
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Product information
| Synonym | BTN3A1; BT3.1; BTF5; BTN3.1; CD277 |
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| Expression Host | HEK293 cells |
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| Tag | C-hFc&His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
