Overview
| Product name | Recombinant Human CD52 Protein |
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| Catalog No. | RP01429 |
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| Description | Recombinant Human CD52 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly25-Ser36) of human CD52 (Accession #NP_001794.2) fused with a Fc, Avi tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CD52 Protein at 5 μg/mL (100 μL/well) can bind SIGLEC10 with a linear range of 20.49-455.65ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | <0.1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 28.98 kDa |
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Background
CD52 / CDW52 is a small glycosylphosphatidylinositol (GPI) anchored glycoprotein. It has a mature peptide comprising only 12 amino acids and is abundantly expressed on human lymphocytes. From the clinical point of view this protein is an important target for therapeutic interventions aimed at leukocyte depletion in hematological malignancies and post-transplant immunosuppression. CD52 / CDW52 may play a role in carrying and orienting carbohydrate. It is an unusually good target for complement-mediated cell lysis.
Product information
| Synonym | CD52; CDW52; EDDM5 |
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| Expression Host | HEK293 cells |
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| Tag | C-hFc&Avi |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
