Recombinant Human CD6 Protein (RP01081)

Product name	Recombinant Human CD6 Protein
Catalog No.	RP01081
Description	Recombinant Human CD6 Protein is produced by HEK293cells expression system. The target protein is expressed with sequence (His18-Glu398) of human CD6 (Accession #NP_006716.3) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its binding ability in a functional ELISA. Immobilized Human CD166 at 1 μg/mL (100 μL/well) can bind Human CD6 with a linear range of 4-389 ng/mL. 2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD6 coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/ml PHA, approximately 60-70% cells will adhere specifically after 60 minutes at 37℃.
Purity	> 92% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	67.36 kDa

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.