제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Human CD6 mAb (A24325)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 HeLa cells (negative control, left) and MOLT-4 (right) cells were surface-stained with ABflo® 647 Rabbit anti-Human CD6 mAb(A24325, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 MOLT-4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD6 mAb(A24325, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Human CD6 mAb
Catalog No.	A24325
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC62124-ABflo647

Background

This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis (PMID: 19525953, 21849685). Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 18-364 of human CD6 (NP_006716.3).
Sequence	HPSPAPPDQLNTSSAESELWEPGERLPVRLTNGSSSCSGTVEVRLEASWEPACGALWDSRAAEAVCRALGCGGAEAASQLAPPTPELPPPPAAGNTSVAANATLAGAPALLCSGAEWRLCEVVEHACRSDGRRARVTCAENRALRLVDGGGACAGRVEMLEHGEWGSVCDDTWDLEDAHVVCRQLGCGWAVQALPGLHFTPGRGPIHRDQVNCSGAEAYLWDCPGLPGQHYCGHKEDAGAVCSEHQSWRLTGGADRCEGQVEVHFRGVWNTVCDSEWYPSEAKVLCQSLGCGTAVERPKGLPHSLSGRMYYSCNGEELTLSNCSWRFNNSNLCSQSLAARVLCSASR
Gene ID
Swiss Prot
Synonyms	CD6; TP120; CD6 molecule
Calculated MW	58kDa/60kDa/63kDa/64kDa/68kDa/71kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, secreted, single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

ABflo® 647 Rabbit anti-Human CD6 mAb images

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD6 mAb (A24325)

Flow cytometry:1X10^6 MOLT-4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD6 mAb(A24325, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.