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HRP-conjugated Mouse anti-Rabbit IgG (H+L) (AS079)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Western blot - HRP-conjugated Mouse anti-Rabbit IgG (H+L) (AS079)

Western blot analysis of lysates from HeLa cells, using β-Actin (AC026) antibody as the primary antibody at dilution of 1:10000.
Secondary antibody: using HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG (H+L) antibody (AS079)) at 1:5000-1:10000 dilution.

Overview

Product nameHRP-conjugated Mouse anti-Rabbit IgG (H+L)
Catalog No.AS079
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW36kDa
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:20000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular location
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HRP-conjugated Mouse anti-Rabbit IgG (H+L) (AS079)}

    Western blot - HRP-conjugated Mouse anti-Rabbit IgG (H+L) (AS079)

    Western blot analysis of lysates from HeLa cells, using β-Actin (AC026) antibody as the primary antibody at dilution of 1:10000.
    Secondary antibody: using HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG (H+L) antibody (AS079)) at 1:5000-1:10000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS079? Please let us know so that we can cite the reference in this datasheet.