Overview
| Product name | Recombinant Human Cyr61/CCN1 Protein |
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| Catalog No. | RP00353 |
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| Description | Recombinant Human Cyr61/CCN1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr25-Asp381) of human Cyr61/CCN1 (Accession #O00622) fused with a hFc tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Recombinant Human Cyr61/CCN1 Fc Chimera immobilized at 1.5 μg/mL (100 μL/well), will mediate >25% BALB/3T3 cell adhesion (3 x 104 cell/well added).
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 65.40 kDa |
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Background
The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation.
Product information
| Synonym | Cellular communication network factor 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Cyr61; CCN1; GIG1 |
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| Expression Host | HEK293 cells |
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| Tag | C-hFc |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
