Recombinant Human EphA2/ECK Protein (RP00024)

Overview

Product name	Recombinant Human EphA2/ECK Protein
Catalog No.	RP00024
Description	Recombinant Human EphA2/ECK Protein is produced by insect cell-baculovirus expression system. The target protein is expressed with sequence (Asp596-Ile976) of human EphA2 (Accession #NP_004422.2).
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Human EphA2 at 1 μg/mL (100 μL/well) can bind Human EFNA1 with a linear range of 4.883 ng/mL-9.206 μg/mL.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	42.94 kDa

Eph receptor A2 (Ephrin type-A receptor 2 or EphA2) is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family.The receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration.

Product information

Synonym	ARCC2; CTPA; CTPP1; CTRCT6; ECK; EPHA2
Expression Host	Baculovirus-Infected Sf9 Cells
Tag	No tag
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.