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Phospho-EPHA2-S897 Rabbit mAb (AP1516)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from NIH/3T3 cells using Phospho-EPHA2-S897 (AP1516) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Phospho-EPHA2-S897 Rabbit mAb (AP1516)

Confocal imaging of MDA-MB-231 cells (treated with FBS) and MDA-MB-231 cells (untreated) using Phospho-EPHA2-S897 Rabbit mAb (AP1516, at dilution of 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of NIH-3T3 cells (treated with FBS) and NIH-3T3 cells (untreated) using Phospho-EPHA2-S897 Rabbit mAb (AP1516, at dilution of 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name	Phospho-EPHA2-S897 Rabbit mAb
Catalog No.	AP1516
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC64633

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S897 of human EPHA2 (NP_004422.2).
Sequence	RLPSTS
Gene ID
Swiss Prot
Synonyms	ECK; CTPA; ARCC2; CTPP1; CTRCT6; Phospho-EPHA2-S897
Calculated MW	108kDa
Observed MW	130KD

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	NIH/3T3 treated by FBS
Cellular location	Cytoplasm, Nucleus, Plasma membrane